The ability to detect and quantify apoptosis, to understand its biochemistry, and to identify its regulatory genes and proteins is crucial to biomedical research. In Apoptosis: Methods and Protocols, Second Edition, expert researchers describe the techniques to best investigate the critical steps involved in the apoptotic process. Presented from several different research perspectives, the volume contains sections covering detection of apoptosis, detection of non-apoptotic cell death, modifications of apoptotic proteins during apoptosis, the analysis of its major regulators, as well as analysis of apoptosis in different organs and in model organisms. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Apoptosis: Methods and Protocols, Second Edition constitutes a key technical reference to the significant methodologies used in the field, and offers beginners and experienced researchers powerful tools to illuminate the phenomena of programmed cell death. TOC:Analyzing Caspase Activation and Caspase Activity in Apoptotic Cells.- Flow Cytometry-Based Apoptosis Detection.- Live to Dead Cell Imaging.- Detection of Apoptosis in Tissue Sections.- Detection of Apoptosis in Cell-Free Systems.- Methods to Analyze Cellular Necroptosis.- Detection of Cell Death by Autophagy.- Methods to Analyze Transglutamination of Proteins Involved in Apoptosis.- Methods to Analyze S-nitrosylation of Proteins Involved in Apoptosis.- Application of in vivo EPR for Tissue pO2 and Redox Measurements.- Assays to Measure p53-dependent and Independent Apoptosis.- Measurement of Changes in Cdk2 and Cyclin O-Associated Kinase Activity in Apoptosis.- Fluorimetric Methods for Detection of Mitochondrial Membrane Permeabilization in Apoptosis.- Regulation of Apoptosis by the Unfolded Protein Response.- Detection of Uncoupling Protein-2 (UCP-2) as a Mitochondrial Modulator of Apoptosis.- A Multiple Approach to Analyzing the Role of MicroRNAs in Apoptosis.- Assessment of Apoptotic Cell Phagocytosis by Macrophages.- Detection of Apoptosis in Mammalian Development.- Detection of Apoptosis in the Central Nervous System.- Genetic Mapping of Anti-apoptosis Pathways in Myeloid Progenitor Cells.- Analysis of Apoptosis in Isolated Primary Cardiac Myocytes.- Cell Death in Myoblasts and Muscles.- Reliable Method for Detection of Programmed Cell Death in Yeasts.- Detection of Cell Death in Drosophila.- Detecting Apoptotic Cells and Monitoring Their Clearance in the Nematode Caenorhabditis elegans.- Detection of Herpes Simplex Virus Dependent Apoptosis.
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Titolo: Apoptosis
Autore:
Editore: Humana Press
Data di Pubblicazione: 2009
Pagine: 399
Formato: Hardcover
ISBN: 9781603270168